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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, <t>FD-FLIM</t> for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.
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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, <t>FD-FLIM</t> for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.
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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, <t>FD-FLIM</t> for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.
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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, <t>FD-FLIM</t> for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.
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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, <t>FD-FLIM</t> for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.
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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, <t>FD-FLIM</t> for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.
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Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, FD-FLIM for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.

Journal: iScience

Article Title: Exploring roles of neuroglobin in oxygen homeostasis and neurodevelopment based on human cerebral organoid models

doi: 10.1016/j.isci.2026.114997

Figure Lengend Snippet: Experimental workflow and impact of NGB silencing on cerebral organoids development Top: Schematic timeline illustrating the stages of cerebral organoid generation from human-induced pluripotent stem cells (hiPSCs), including embryoid body (EB) formation, neural induction, fusion and expansion, and maturation. All measurement points for data analysis were denoted. Bottom: Experimental workflow comparing control hiPSC-derived cerebral organoids with NGB -silenced organoids. Organoids were analyzed using single-cell RNA sequencing (scRNA-seq) for genotypic profiling, FD-FLIM for functional analysis, confocal microscopy for structural assessment, and microelectrode array (MEA) recordings for functional evaluation.

Article Snippet: The microscope was equipped with a high-power LED excitation source (470 nm nominal wavelength; Thorlabs, M470LP-C2) and a dual-tap CMOS FLIM camera (PCO.FLIM, Excelitas Technologies Corp., Waltham, MA).

Techniques: Control, Derivative Assay, Single Cell, RNA Sequencing, Functional Assay, Confocal Microscopy, Microelectrode Array